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ACS 基因家族序列搜索，比对，进化分析 两种分析策略： 1 序列搜索 先搜索相关基因，找到一条 ACS 蛋白序列，进行在线比对，把 evalue 值高、query cover 高于 88%的序列下载，命名为 ACS_family.fa，上传至服务器，进行后续分析； 2 下载四个物种蛋白组数据 通过服务器进入 NCBI 数据库，将水稻，高梁，深山南芥，马铃薯 四个物种蛋白组序列下载到本地，上传至服务器，分别命名为 oryza.fa，sorghum.fa，arabidopsis.fa，solanum.fa； 2 与各个物种进行比对 比对：先对四个物种建库，分别与 ACS_family.fa 进行比对，结果 输出文件分别为 ACS_AL、ACS_OS、ACS_ST、ACS_Sorghum （结果每条序列比对只输出前 10 条）； 3 比对结果序列下载作树 比对结果整合到 Excel（ACS_blast_results），NCBI 在线下载表中 所有序列，命名为 ACS_prot.fasta，所有序列比对做进化树。 NJ tree XP_***2.1_probable_aminotransferase_TAT2_Sorghum_bicolor XP_***1.1_probable_aminotransferase_TAT2_Sorghum_bicolor XP_***6.1_probable_aminotransferase_TAT2_Oryza_sativa_Japonica_Group XP_***2.1_nicotianamine_aminotransferase_A_Sorghum_bicolor XP_***2.2_nicotianamine_aminotransferase_A_Oryza_sativa_Japonica_Group XP_***8.1_nicotianamine_aminotransferase_A_isoform_X1_Oryza_sativa_Japonica_Group XP_***9.1_nicotianamine_aminotransferase_A_isoform_X2_Oryza_sativa_Japonica_Group XP_***4.1_tyrosine_aminotransferase_isoform_X2_Oryza_sativa_Japonica_Group XP_***5.1_tyrosine_aminotransferase_isoform_X1_Sorghum_bicolor XP_***3.1_nicotianamine_aminotransferase_A_Sorghum_bicolor XP_***4.1_nicotianamine_aminotransferase_A-like_Sorghum_bicolor XP_***5.1_probable_aminotransferase_TAT2_isoform_X1_Sorghum_bicolor XP_***6.1_probable_aminotransferase_TAT2_isoform_X2_Sorghum_bicolor XP_***9.1_uncharacterized_protein_LOC***6_Sorghum_bicolor XP_***6.1_bifunctional_aspartate_aminotransferase_and_glutamate/aspartate-prephenate_aminotransferase_Sorghum_bicolor XP_***2.1_bifunctional_aspartate_aminotransferase_and_glutamate/aspartate-prephenate_aminotransferase_Oryza_sativa_Japonica_Group XP_***9.2_bifunctional_aspartate_aminotransferase_and_glutamate/aspartate-prephenate_aminotransferase_Sorghum_bicolor XP_***2.1_glutamate--glyoxylate_aminotransferase_2_Oryza_sativa_Japonica_Group XP_***8.1_glutamate--glyoxylate_aminotransferase_1-like_isoform_X2_Sorghum_bicolor XP_***0.1_alanine_aminotransferase_2_isoform_X1_Oryza_sativa_Japonica_Group XP_***1.1_alanine_aminotransferase_2_isoform_X3_Oryza_sativa_Japonica_Group XP_***0.1_alanine_aminotransferase_2_isoform_X2_Oryza_sativa_Japonica_Group XP_***4.1_alanine_aminotransferase_2_isoform_X3_Sorghum_bicolor XP_***9.1_alanine_aminotransferase_2_isoform_X4_Sorghum_bicolor XP_***1.1_alanine_aminotransferase_2_isoform_X2_Sorghum_bicolor XP_***9.1_alanine_aminotransferase_2_isoform_X3_Oryza_sativa_Japonica_Group XP_***0.2_alanine_aminotransferase_2_isoform_X1_Oryza_sativa_Japonica_Group XP_***9.1_alanine_aminotransferase_2_isoform_X2_Oryza_sativa_Japonica_Group XP_***8.1_alanine_aminotransferase_2_Oryza_sativa_Japonica_Group XP_***4.1_alanine_aminotransferase_2_isoform_X1_Sorghum_bicolor XP_***1.1_alanine_aminotransferase_2_isoform_X1_Sorghum_bicolor XP_***9.1_alanine_aminotransferase_2_isoform_X2_Sorghum_bicolor XP_***1.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase-like_isoform_X2_Solanum_tuberosum XP_***8.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase-like_isoform_X1_Solanum_tuberosum XP_***0.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase-like_Solanum_tuberosum NP_***7.1_1-aminocyclopropane-1-carboxylate_synthase_Solanum_tuberosum XP_***9.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase-like_Solanum_tuberosum XP_***0.1_1-aminocyclopropane-1-carboxylate_synthase_6_Arabidopsis_lyrata_subsp._lyrata XP_***6.1_1-aminocyclopropane-1-carboxylate_synthase-like_protein_1_Arabidopsis_lyrata_subsp._lyrata XP_***2.1_1-aminocyclopropane-1-carboxylate_synthase_2_Arabidopsis_lyrata_subsp._lyrata XP_***9.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_2_Solanum_tuberosum XP_***9.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_4_Solanum_tuberosum XP_***4.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_4-like_Solanum_tuberosum XP_***2.1_1-aminocyclopropane-1-carboxylate_synthase_Sorghum_bicolor XP_***7.1_1-aminocyclopropane-1-carboxylate_synthase_Oryza_sativa_Japonica_Group XP_***9.1_1-aminocyclopropane-1-carboxylate_synthase_7_Sorghum_bicolor XP_***7.1_1-aminocyclopropane-1-carboxylate_synthase_7_Oryza_sativa_Japonica_Group XP_***2.1_1-aminocyclopropane-1-carboxylate_synthase_7_Oryza_sativa_Japonica_Group XP_***3.1_1-aminocyclopropane-1-carboxylate_synthase_7_Oryza_sativa_Japonica_Group XP_***1.1_PREDICTED:_LOW_QUALITY_PROTEIN:_1-aminocyclopropane-1-carboxylate_synthase_7_Solanum_tuberosum XP_***3.1_1-aminocyclopropane-1-carboxylate_synthase_7_Arabidopsis_lyrata_subsp._lyrata XP_***0.1_1-aminocyclopropane-1-carboxylate_synthase_1_Sorghum_bicolor XP_***5.1_1-aminocyclopropane-1-carboxylate_synthase_1_Oryza_sativa_Japonica_Group XP_***9.1_1-aminocyclopropane-1-carboxylate_synthase_11_Arabidopsis_lyrata_subsp._lyrata XP_***7.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_3_Solanum_tuberosum XP_***9.1_1-aminocyclopropane-1-carboxylate_synthase_4_Arabidopsis_lyrata_subsp._lyrata XP_***9.1_1-aminocyclopropane-1-carboxylate_synthase_8_Arabidopsis_lyrata_subsp._lyrata XP_***9.1_1-aminocyclopropane-1-carboxylate_synthase_5_Arabidopsis_lyrata_subsp._lyrata XP_***0.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_3-like_Solanum_tuberosum XP_***6.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_3_Solanum_tuberosum XP_***0.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_3-like_Solanum_tuberosum XP_***1.1_PREDICTED:_1-aminocyclopropane-1-carboxylate_synthase_3-like_Solanum_tuberosum XP_***5.1_3-methyl-2-oxobutanoate_hydroxymethyltransferase_2_mitochondrial_Oryza_sativa_Japonica_Group XP_***4.1_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1_mitochondrial_Sorghum_bicolor XP_***5.1_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1_mitochondrial_Sorghum_bicolor XP_***6.1_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1_mitochondrial_Sorghum_bicolor XP_***0.1_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1_mitochondrial_Oryza_sativa_Japonica_Group XP_***8.1_3-methyl-2-oxobutanoate_hydroxymethyltransferase_2_mitochondrial_Arabidopsis_lyrata_subsp._lyrata XP_***5.1_PREDICTED:_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1_mitochondrial-like_Solanum_tuberosum XP_***9.1_LOW_QUALITY_PROTEIN:_3-methyl-2-oxobutanoate_hydroxymethyltransferase_1_mitochondrial_Arabidopsis_lyrata_subsp._lyrata NJ tree XP ***9.1 PREDICTED: 1-aminocyclopropane-1-carboxylate synthase 4 Solanum tuberosum XP ***4.1 PREDICTED: 1-aminocyclopropane-1-carboxylate synthase 4-like Solanum 339629.1 PREDICTED: 1-aminocyclopropane-1-carboxylate synthase 2 SoXlaPn0u0m28tu9b2e0r6o2s.u1m1-aminocyclopropane-1-carboxylate synthase 2 Arabidopsis lyrata subsp. lyrata -carboxylate synthase-like protein 1 Arabidopsis lyrata subsp. lyrata ymethyltransferase 1 mitochondrial Arabidopsis lyrata subsp. l thyltransferase 1 mitochondrial-like Solanum tuberosum a8m37in2o.c1y1c-loapmroinpoacnyec-lo1p-croaprbaonxey-l1a-tceasrybnotxhyalasteeOsyrynzthaassaetivSaorJgahpuomnicbaicGolroorup ssee 21 mmiittoocchhoonnddrriiaallAOrraybziadospastiivsalyJraaptaonsiucbasGp.rolyurapta ransferase 1 mitochondrial Sorghum bicolor se TAT2 Sorghum bicolor yraltrnasnfesrfearsaes1e m1 imtoictohcohnodnrdiarliaSloSrogrhguhmumbicboicloorlor T2 Sorghum bicolor tiva Japonica Group XP 0028X72P507006.136N10-Pa7m3090in.11o2cP7y5RXc3PlEo6Dp70rI.0oC16pT13aE-4naD3em9:-13i1n-0-oca.ca1myrPbcinolRooxpEcyrDyloacIptlCeoaTpnsrEeyon-Dp1t:ha-c1ana-seare-bm1o6-icxnAayorlcaabytboecixdlsoyoylpapnrtsotehipsasaslynynereath-St1ao-slcsaeaun-rblubiksmoepx.tySullyaobrtleaertnaosus XP ***1.1 PREDICTED: 1-aminocyclopropane-1-c XP ***8.1 PREDICTED: 1-aminocyclopropan XP ***1.1 PREDICTED: LOW QUALITY PROTEIN: 1-aminocyclop XXPP***3793 内容过长，仅展示头部和尾部部分文字预览，全文请查看图片预览。 XP_***4.1_Oryza_sativa_Japonica_Group XP_***1.1_Oryza_sativa_Japonica_Group XP_***6.1_Oryza_sativa_Japonica_Group XP_***4.1_Sorghum_bicolor XP_***5.1_Sorghum_bicolor XP_***5.1_Oryza_sativa_Japonica_Group XP_***6.1_Sorghum_bicolor XP_***0.1_Oryza_sativa_Japonica_Group XP_***5.1_Solanum_tuberosum XP_***8.1_Arabidopsis_lyrata_subsp._lyrata XP_***9.1_Arabidopsis_lyrata_subsp._lyrata XP_***5.1_Arabidopsis_lyrata_subsp._lyrata Bayes tree [文章尾部最后500字内容到此结束,中间部分内容请查看底下的图片预览]请点击下方选择您需要的文档下载。

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